MCD Peptide
- 貨號 Cat. No.
- M-250
- 規格 Size
- 0.1 mg 5x0.1mg
Description
MCD Peptide
A Potent Blocker of Kv1.2 Channels
| Product#: | M-250 |
| Sizes: |
| 0.1 mg |
| 5 x 0.1 mg |
| M.W.: | 2588.2 |
| Sequence: | IKCNCKRHVIKPHICRKICGKN |
| Purity: | >98% |
| Bioassay: |
Inhibition of Kv1.2 channels expressed in Xenopus oocytes by MCD peptide. |
 |
Legend: Potent inhibition of Kv1.2 channel currents by 10 nM of MCD peptide. Left: Time course of current inhibition by 10 nM MCD peptide and recovery in Xenopus oocytes expressing Kv1.2 channels. Holding potential was -100 mV and test pulse to 0 mV was delivered every 10 seconds. The bar represents period of toxin perfusion. Right: Superimposed traces of Kv1.2 channel current from the experiment shown on the left before (black) and during bath application of 10 nM MCD peptide. |
| References: |
1. | Jentsch, J. and Mucke, H.W. (1977) Int. J. Pept. Protein Res. 9, 78. |
2. | Schwartz, L. B. et al. (1979) J. Immunol. 123, 1445. |
3. | Aniksztejn, L. et al. (1990) Neuroscience 35, 63. |
4. | Guillemare, E. et al. (1992) Biochemistry 31, 12463. |
5. | Grissmer, S. et al. (1994) Mol. Pharmacol. 45, 1227. |
6. | Southan, A. P. and Owen, D. G. (1997) British J. Pharmacol. 122, 335. |
7. | Mourre, C. et al. (1997) Brain Res. 762, 223. |
MCD peptide (Mast Cell Degranulating Peptide) is a 22 amino acid peptidyl toxin isolated from Apis mellifera bee venom.1
MCD peptide induced histamine release from mast cells2 and release of glutamate in CA1 region of rat hippocampus in a dose dependent manner.3
MCD peptide was shown to block KV1.2 channels in Xenopus oocytes4 and to inhibit KV1.1 (IC50 490 nM) and KV1.2 (IC50 440 nM) expressed in cell lines while having no effect on KV1.3, KV1.5 and KV3.1.5 In accordance, MCD peptide (6.4mM) enhanced the magnitude of field potentials in CA1 region as recorded in hippocampal slices6 and induced epileptiform seizures and brain damage after intracerebroventricular injection of (200 pM) in rats.7