MAPKAPK2 / MK2 Antibody (LS-B2680)

貨號 Cat. No.: LS-B2680
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2680
Antibody Validation:	This antibody replaces catalog number LS-C25415. It has been validated for use in the following assays: IHC-P.
Antibody Target:	MAPKAPK2 / MK2
Synonyms:	MAPKAPK2 Antibody, MAP KAP Kinase 2 Antibody, map kinase-activated protein kinase 2 Antibody, MAPK-activated protein kinase-2 Antibody, MAPKAP Kinase-2 Antibody, MAPKAPK-2 Antibody, mitogen-activated protein kinase-activated protein kinase 2 Antibody, MK2 Antibody
Family:	Protein Kinase
Subfamily:	MAPKAPK
Host:	MAPKAPK2 antibody was produced in Rabbit
Antigen Species:	MAPKAPK2 antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	MAPKAPK2 antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr222 of human MAPKAPK-2.
Antibody Specificity:	Detects endogenous levels of Thr222 phosphorylated MAPKAPK-2. It may cross-react with Thr200 phosphorylated MAPKAPK-3 and Thr182 phosphorylated MAPKAPK-5. Species cross-reactivity: Human, mouse and rat.
Antigen Modification:	pThr222
Predicted Species Reactivity:	Mouse, Rat
Purification:	Protein A Column
Presentation:	10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2680 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2680 was determined to be 1:100.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (1:100), Western blot (WB) (1:1000), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µl