Proteins and Peptides
MAP3K1 / MEKK1 Antibody (LS-B2524)
- 貨號 Cat. No.
- LS-B2524
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2524 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C16496. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | MAP3K1 / MEKK1 |
| Synonyms: | MAP3K1 Antibody, MAP/ERK kinase kinase 1 Antibody, MAPKKK1 Antibody, mek kinase 1 Antibody, MEKK Antibody, MEKK1 Antibody |
| Family: | Protein Kinase |
| Subfamily: | MAP3K |
| Host: | MAP3K1 antibody was produced in Rabbit |
| Antigen Species: | MAP3K1 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Recombinant protein |
| Immunogen: | MAP3K1 antibody was raised against recombinant protein encompassed within amino acids 130-300 of the human MEKK1 protein. |
| Antibody Specificity: | Reacts specifically with MEKK1 protein detecting a monomer species migrating at ~69kD and a trimer species migrating at ~200kD. Cross-reactivity with other proteins has not been observed. Species cross-reactivity: human MEKK1. Tested by Western blotting with HeLa, 293 (kidney) and MEKK1 transfected cells. |
| Antigen Modification: | aa130-300 |
| Purification: | Affinity Chromatography |
| Presentation: | PBS, pH 7.4, 0.1% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2524 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2524 was determined to be 5 ug/ml. Western Blot: 1-3 ug/ml. Tested using HeLa 293 and MEKK1 cells. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB), ELISA (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.25 mg/ml |
同品牌其他產品
