LY 294002
- 貨號 Cat. No.
- L-300
- 規格 Size
- 10 x 0.25 mg 1 mg 5mg 5x5mg
Description
LY 294002
A Specific Inhibitor of PI 3-Kinase
| Product#: | L-300 |
| Sizes: |
| 10 x 0.25 mg |
| 1 mg |
| 5 mg |
| 5 x 5 mg |
| M.W.: | 307.35 |
| CAS No.: | [154447-36-6] |
| Structure: |
| . |
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| Purity: | >99% |
| Bioassay: |
| The biological activity was tested to confirm the ability of LY 294002 to inhibit PI 3 - Kinase.
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NIH/3T3 cells were preincubated for 30 min in different concentrations of LY294002 or Wortmannin, |
| References: |
1. | Kapeller, R. et al. (1994) Bioessays 16, 565. |
2. | Duronio, V. et al. (1998) Cell Signal. 10, 233. |
3. | Vlahos, C.J. et al. (1994) J. Biol. Chem. 269, 5241. |
4. | Vlahos, C.J. et al. (1995) J. Immunol. 154, 2413. |
5. | Curnock, A.P. et al. (1998) Cell. Immunol. 187, 77. |
6. | Nicholson, K.M. et al. (2003) Cancer Lett. 190, 31. |
7. | Coffer, P.J. et al. (1998) Biochem. J. 335 ( Pt 1), 1. |
8. | Shao, J. et al. (2004) Cancer Res. 64, 229. |
9. | Vivanco, I. et al. (2002) Nat. Rev. Cancer 2, 489. |
PI 3-kinase is an enzyme implicated in growth factor signal transduction by associating with receptor and non-receptor tyrosine kinases. 1, 2 LY-294002 is a potent and specific cell-permeable inhibitor of Pl 3-kinase that acts on the ATP-binding site of the enzyme.3
In in vitro assays it displays an IC50 of 1.4 然 on purified PI 3-kinase, but did not inhibit PKC, PKA, MAP kinase and PI 4-kinase.3 In fMet-Leu-Phe stimulated neutrophils, LY-294002 completely abolished PI 3-kinase activity at 50 然.4 LY-294002 induces apoptosis in many cell types by blocking the PI3-K/Akt anti-apoptotic pathway.5, 6 Thus LY-294002 is useful tool for identifying the biochemical events regulated by the PI3-K/Akt pathway.7 This pathway has important relevance to the cellular events involved in cancer.6, 8, 9