LR3IGF-I Rapid ELISA Kit: Human (2 plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Human LR3 insulin-like Growth Factor-I.

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : LR³IGF-I is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1524

 

Description (Long Description) : 

Human LR³ insulin-like Growth Factor-I (LR³IGF-I) was developed by GroPep Bioreagents (www.gropep.com) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR³IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR³IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis.

This LR³IGF-I Rapid ELISA kit combines GroPep€™s many years of expertise in the field of IGF research and Biosensis€™ newly established Rapid ELISA platform. This collaboration has resulted in the new LR³IGF-I Rapid ELISA kit, which provides for the sensitive, specific and reliable quantification of LR³IGF-I protein in less than 3 hours!
 
The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR³IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR³IGF-I capture antibody pre-coated onto an ELISA plate, standard LR³IGF-I protein, a biotinylated anti- LR³IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR³IGF-I present in samples and the supplied protein standard.

This LR³IGF-I Rapid™ ELISA kit has been developed, optimized and validated to quantify LR³IGF-I protein in cell culture medium. It is likely to be used to measure LR³IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

12 months from purchase.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :Recombinant LR3-IGF1 protein, made in E.coli

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.

 

MSDS (File name) :