Live/Dead Cell Viability Assay Kit (for Mammalian Cells)

Alternate Name : Cell viability assay kit

Tag Line :

Easy, non-radioactive assay kit to measure cell viability/proliferation, cytotoxicity and apoptosis by microscopy/FACS analysis.

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Fluorescent microscopy or FACS analysis (Ex/Em 485/530 nm) to detect stained live cells; (Ex/Em 495/635) to detect stained dead cells
• Species reactivity- Mammalian
• Applications- Stained live and dead cells can easily be visualized by fluorescence microscopy or FACS analysis.

Detection Method: Detection method- Fluorescent microscopy or FACS analysis (Ex/Em 485/530 nm) to detect stained live cells; (Ex/Em 495/635) to detect stained dead cells

Features & Benefits:

Easy to use Protocol
Non-radioactive detection. Detect using either microscopy or FACS.
Measure Cell Proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis

Description:

Quantification of number of live and dead cells is an indispensable tool in cell biology research. BioVision’s Live/Dead Cell Viability Assay Kit, provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes. Live cell dye easily penetrates intact, live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm. Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells. This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.