Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit
Kits
- 貨號 Cat. No.
- K739-100
- 規格 Size
- K739-100
- Sample Type
- Cell and tissue culture supernatants 、 plasma and other biological fluids (optimized by end user)
Description
Alternate Name :
Tag Line :
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method-Absorbance (532 nm) or Fluorescence (Ex/Em 532/553 nm)
• Species reactivity- All
• Applications- The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
Detection Method:
Features & Benefits:
• Simple procedure
• Fast and convenient
• Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples
Description:
Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. BioVision’s Lipid Peroxidation Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (λ = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.