Leptin, rat recombinant

Alternate Names / Synonyms: OB Protein, Obesity Protein, OBS, Obesity factor.

Gene Symbol: LEP

Accession #: P50596

Gene ID: 25608

Gene Source: Rat

Recombinant Host: E. coli

Appearance: Lyophilized protein

Physical Form: Lyophilized from 4.5 µM NaHCO3.

Molecular Weight: 16.2 kDa

Purity by SDS-PAGE: ≥95%

Purity by HPLC: ≥95%

Endotoxin Level: <0.1 ng/μg

Biological Activity: The EDâ‚…â‚€ determined by the leptin-dependent stimulation of human OB-R transfected murine BaF3 indicator cells is 0.35-0.06 ng/ml.

Reconstitution: Reconstitute in water to a concentration of 0.1–1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for three months. For long-term storage it is recommended to add a carrier protein (0.1% HAS or BSA).

Storage Temp.: -20°C

Shipping: Gel pack

Background Information: Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body’s fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Rat Leptin is a 16.2 kDa protein containing 147 amino acid residues.

Handling: Centrifuge the vial prior to opening.