Leptin (Rat) ELISA Kit

Alternate Name :

Tag Line :

A Sandwich ELISA kit for the quantitative measurement of Leptin in rat serum, plasma, culture supernatants and other biological fluids.

Storage Conditions: 4°C

Shelf life: 6 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Rat
• Application- quantitative measurement of Leptin in serum, plasma, tissue homogenates and other biological fluids.

Detection Method: Absorbance (450 nm)

Features & Benefits:

Easy, convenient and time-saving method to measure the level of Leptin in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Detection Range: 0.156 - 10 ng/ml
Sensitivity: < 0.094 ng/ml
Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
No significant cross-reactivity or interference was observed.

Description:

Leptin, the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. In obesity, a decreased sensitivity to leptin occurs, resulting in an inability to detect satiety despite high energy stores. BioVision’s Leptin ELISA kit is a sandwich ELISA assay for the quantitative measurement of Leptin in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Leptin captured from the samples.