Leptin, murine recombinant

Alternate Names / Synonyms: OB Protein, Obesity Protein, OBS, Obesity factor.

Gene Symbol: LEP

Accession #: P41160

Gene ID: 16846

Gene Source: Mouse

Recombinant Host: E. coli

Appearance: Lyophilized protein

Physical Form: Lyophilized with no additives

Molecular Weight: 16.2 kDa

Purity by SDS-PAGE: ≥98%

Purity by HPLC: N/A

Endotoxin Level: <0.1 ng/μg

Biological Activity: Determined in an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 mg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.

Reconstitution: Reconstitute in 10 mM HCl to a concentration of 1 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or –20oC for future use.

Storage Temp.: -20°C

Shipping: Gel pack

Background Information: Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body’s fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Murine Leptin is a 16 kDa protein containing 147 amino acid residues.

Handling: Centrifuge the vial prior to opening.