Proteins and Peptides
LCP1 Antibody (LS-B2608)
- 貨號 Cat. No.
- LS-B2608
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2608 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C26110. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | L-plastin (LCP1) |
| Synonyms: | LCP1, CP64, DKFZp781A23186, FLJ25423, FLJ26114, FLJ39956, l-plastin, LC64P, LCP-1, LPL, Plastin L, PLS2 |
| Host: | LCP1 antibody was produced in Mouse |
| Antigen Species: | LCP1 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antigen Type: | Recombinant protein |
| Immunogen: | LCP1 antibody was raised against human recombinant L-plastin protein. |
| Antibody Specificity: | Recognizes LPL in Ca2+ independent fashion. Does not cross-react with T-or I-plastin. Species cross-reactivity: Human. Does not cross react with mouse. |
| Non-reactive Species: | Mouse |
| Purification: | Protein G Column |
| Presentation: | 10 mM PBS, pH 7.4. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2608 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2608 was determined to be 10 ug/ml. Western Blot: 1-2 ug/ml for 2 hours at RT. Immunofluorescence: Fixed in methanol or 3% paraformaldehyde. Immunoprecipitation (Native): 2 ug/mg protein lysate using Protein G. Positive control: Human polymorphonuclear neutrophils and monocytes, MCF-7, Jurkat cells. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1 - 2 µg/ml), Immunoprecipitation (IP), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 1 mg/ml |
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