LC3A/B Antibody

Antibodies

貨號 Cat. No.
4108S
規格 Size
100 µg
品牌 Brand
Cell Signaling Technology
Reactivity
Human 、 Mouse 、 Rat

Description




Product Usage Information
























Application



Dilution



Western Blotting



1:1000



Immunofluorescence (Immunocytochemistry)



1:200



Flow Cytometry



1:50




Storage


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.


Protocol


Specificity / Sensitivity


LC3A/B Antibody detects endogenous levels of total LC3A and LC3B proteins. Cross-reactivity may exist with LC3C. Stronger reactivity is observed with the type II form of LC3A/B.


Species Reactivity:


Human, Mouse, Rat


Species predicted to react based on 100% sequence homology:


Monkey, Chicken, Xenopus, Zebrafish, Dog


Source / Purification


Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly40 of LC3B. Antibodies are purified by protein A and peptide affinity chromatography.





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