Proteins and Peptides
Lamin C Antibody (LS-B2972)
- 貨號 Cat. No.
- LS-B2972
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2972 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C82209. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Lamin C |
| Host: | Lamin C antibody was produced in Rabbit |
| Antigen Species: | Lamin C antibody was raised against Human |
| Clonality: | Polyclonal |
| Antigen Type: | Synthetic peptide |
| Immunogen: | Lamin C antibody was raised against a peptide made from the last 8 amino acids of Lamin C, including an N-terminal lysine as a linker (KHHVSGSRR), coupled to KLH |
| Antibody Specificity: | This antibody reacts strongly in immunoblotting with fragments corresponding to peptide 319-572aa of Lamin C. When tested against nuclear protein extracts from HeLa cells it reacts with a single band migrating at 65 kD. Stains Lamin C from human, mouse and rat material |
| Antigen Modification: | N-Terminus |
| Predicted Species Reactivity: | Mouse, Rat |
| Purification: | Affinity Chromatography |
| Presentation: | PBS, 0.1% sodium azide. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2972 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2972 was determined to be 1:50. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:50), Western blot (WB), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
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