Lactate Colorimetric/Fluorometric Assay Kit

Kits

貨號 Cat. No.
K607-100
規格 Size
K607-100
Sample Type
as well as many other biological fluids 、 Cell and tissue culture supernatants 、 Plasma 、 Serum 、 urine

Description




Alternate Name :


 

Tag Line :



 

Storage Conditions: -20°C


 

Shelf life: 12 months


 

Summary :


• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Mammalian
• Applications- The kit can detect 0.001-10 mM of various Lactate samples.


 

Detection Method:


 

Features & Benefits:


• Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of Lactate colorimetrically and fluorometrically


 

Description:


Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate. In the Lactate Assay Kit, lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm). The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples. The kit can detect 0.001-10 mM of various Lactate samples.


 





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