KT5720

KT5720

Selective PKA Inhibitor

Product#:

K-190

Sizes:

50 mg

5 x 50 mg

0.5 mg

KT5720 is a semi-synthetic derivative of K525a. It is a cell-permeable, selective inhibitor  

of cAMP-dependent protein kinase (PKA; IC₅₀=56nM). No significant effect on protein  

kinase C (PKC), protein kinase G (PKG) or myosin light chain kinase (MLCK),  

was observed.¹ KT5720 was used to examine the role of PKA in various cellular events  

such as cell adhesion,² neurite branching,³ anterograde axonal transport,⁴ memory  

consolidation,^5,6 and regulation of transcription by cyclic AMP-dependent response  

element-binding protein (CREB).⁷

M.W.:

537

CAS No.:

[108068-98-0 ]

Structure:

.

Purity:

Greater than 98% as evaluated by HPLC.

Bioassay:

The biological activity was tested to confirm the ability of KT5720 to inhibit PKA.

Western blot analysis of NIH/C6 cells preincubated for 45 min in the presence   or absence of KT5720 and then stimulated with Forskolin (10mM) for 20 min.   The blotted proteins were detected with Anti-Phospho-(Ser/Ther) PKA substrate.

References:

1.	Kase, H. et al. (1987) B.B.R.C. 142, 436.
2.	Mohri, T. et al. (1998) Cell. Struct. Funct. 23, 255.
3.	Audesirk, G. et al. (1997) Brain Res. Dev. Brain Res. 102, 247.
4.	Okada, Y. et al. (1995) J. Neurosci. 15, 3053.
5.	Vianna, M.R. et al. (1999) Behav. Pharmacol. 10, 223.
6.	Huang, Y.Y. et al. (2000) J. Neurosci. 20, 6317.
7.	Kim, M.S. et al. (2002) Biochem. Biophys. Res. Commun. 291, 663.