KSHV/HHV-8 IgG Antibody IFA Kit

Intended Use

Advanced Biotechnologies Inc’s Indirect Fluorescent Assay (IFA) for Human Herpesvirus-8 (HHV-8) antibody is intended for the qualitative and quantitative determination of IgG antibodies to HHV-8 in human serum or plasma. The assay is intended as an aid in the detection of primary, latent, persistent, or reactivated infection with HHV-8 based on serological evidence.

Principle of the Assay

ABI’s HHV-8 IgG IFA kit uses a rapid, simple, sensitive method for determination of IgG antibodies to Human Herpesvirus-8.  ABI’s fluorescent antibody assay utilizes the indirect method of fluorescent antibody staining first described by Weller and Coons in 1954.  The procedure is carried out in two basic reaction steps:

1. Diluted human sera or plasma are added to the wells of IFA slides. During incubation, diluted unknown samples and undiluted control samples are incubated with a mixture of HHV-8 infected/induced cells, which are attached to the slides. Specific HHV-8 antibodies will bind to the antigen present in infected cells, formulating immunological complexes. If the sample being examined contains no antibodies to HHV-8, no complex is formed.  After incubation, the IFA slides are washed to remove all unbound components.


2. Anti-human IgG conjugated to the dye Fluorescein Isothiocyanate (FITC) is added to each well, and during incubation, will bind to the antibody-antigen complexes present on the wells.  After incubation, each slide is washed to remove unbound conjugate.  After mounting, the slides are ready to evaluate under a fluorescent microscope.

If antibodies to HHV-8 are present (a positive reaction), bright apple-green fluorescence will be seen.

Kit Components

1. KSHV/HHV-8 10-well slides (10 slides, KS-1 cells)


2. Positive Control Plasma


3. Negative Control Plasma


4. FITC labeled anti-Human IgG


5. 20X Wash Buffer


6. Mounting Solution


7. IFA Accessory Pack