KSHV/HHV-8 IgG Antibody ELISA Kit

Intended Use

Advanced Biotechnologies Inc’s Indirect Human Herpesvirus-8 (HHV-8) IgG antibody Enzyme Linked ImmunoSorbent Assay (ELISA) is intended for the detection of HHV-8 IgG specific antibodies in human serum or plasma.

8-well strips are coated with optimal amounts of solubilized HHV-8 whole virus extract. The test procedure involves three incubation steps:

1. Tested human samples are added to the microtiter wells and any specific antibodies present to HHV- 8 will bind to the antigen-coated plates, formulating immunological complexes. After the incubation, the strips are washed to remove unbound sample components.


2. Anti-human IgG conjugated to the enzyme horseradish peroxidase (HRP) is added to the wells and during incubation, will bind to the antibody-antigen complexes already present in the wells. After incubation, the wells are washed to remove unbound conjugate.


3. HRP substrate tetramethylbenzidine (TMB) is added to the wells. During incubation, enzyme mediated cleavage of the substrate results in a color change. The reaction is stopped by adding Stop Solution, and the wells with positive samples (i.e., contain antibodies to HHV-8) will change color from blue to yellow. The wells with negative samples (i.e., do not contain antibodies to HHV-8) will remain almost colorless. The color intensity is proportional to the level of HHV-8 IgG antibodies in a sample, and is measured spectrophotometrically.