Kryptopyrrole colorimetric

Kryptopyrroluria is a genetically determined enzymatic disorder of the hem
metabolism, which is essentially compensated under normal conditions.
However, under stress conditions, there is an insufficient compensation and
the disorder is manifested in the form of several different disease symptoms.
Kryptopyrroluria can be diagnosed with high reliability by detection of
abnormal increased pyrrole excretion in urine.
Kryptopyrrole, 2,4 dimethyl-3-ethylpyrrole, is a byproduct of disordered
hemoglobin synthesis. It has been found out that circulating kryptopyrrole
forms a Schiff's base with the aldehyde group of pyridoxal-5’-phosphate (P5-P),
the active vitamin B6 form in the blood, whereby an additional zinc
binding results in formation of an insoluble kryptopyrrole – zinc - vitamin B6 -
complex. Elevated excretion of kryptopyrrole in the urine is associated with
an enhanced vitamin B6 and zinc excretion and as a result in a B6 and zinc
deficiency in the blood.
Kryptopyrroluria is involved in behavior and emotional disorders. Increased
kryptopyrrole values were found in children and juveniles with ADHS
(Attention Deficit Hyperactivity Syndrome), as well as in children with
disturbed short-term memory, learning difficulties, dyslexia and stressintolerance.
Positive therapy experiences were made with substitution of
zinc, vitamin B6 and manganese.
Our colorimetric test is designed for determination of urinary kryptopyrrole.
Urine specimen collection and sample transport are very important for
proper test results. Special urine test tubes for kryptopyrrole determination
can be ordered in addition to the test kit.