Proteins and Peptides
KRT19 Antibody (LS-B2650)
- 貨號 Cat. No.
- LS-B2650
- 規格 Size
- 125 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2650 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C24989. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | KRT19 |
| Synonyms: | KRT19 Antibody, ck 19 Antibody, CK19 Antibody, cytokeratin 19 Antibody, K19 Antibody, K1CS Antibody, keratin, type i cytoskeletal 19 Antibody, krt19 Antibody, MGC15366 Antibody |
| Host: | KRT19 antibody was produced in Mouse |
| Antigen Species: | KRT19 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG2a,l |
| Antigen Type: | Cells |
| Immunogen: | KRT19 antibody was raised against human breast cancer MCF-7 cells. Epitope: aa312-335 Cellular Localization: Cytoplasmic. MW of antigen: 40kD |
| Antibody Specificity: | Recognizes the rod domain of keratin 19 (40kD). Reacts with the MCF-7 cells, which are known to contain keratin 19. It shows no reaction with the cells lacking keratin 19 such as A431 and HeLa. Species cross-reactivity: Human. Weak reactivity with rat, mouse and guinea pig. |
| Antigen Modification: | aa312-335 |
| Predicted Species Reactivity: | Guinea pig, Mouse, Rat |
| Purification: | Protein G Column |
| Presentation: | 10 mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2650 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2650 was determined to be 10 ug/ml. Western Blot: 2-4 ug/ml for 2 hrs at RT. Positive control: LS174T and HT29 cells, skin. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Immunohistochemistry - Frozen (IHC-Fr) (1 - 2 µg/ml), Western blot (WB) (2 - 4 µg/ml), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher) |
| Size: | 125 µl |
| Concentration: | 0.1 mg/ml |
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