KRT14 / Cytokeratin 14 Antibody (LS-B2443)

貨號 Cat. No.: LS-B2443
規格 Size: 125 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2443
Antibody Validation:	This antibody replaces catalog number LS-C24972. It has been validated for use in the following assays: IHC-P.
Antibody Target:	KRT14 / Cytokeratin 14
Synonyms:	KRT14 Antibody, CK14 Antibody, EBS3 Antibody, EBS4 Antibody, epidermal type i keratin [fragment] Antibody, K14 Antibody, keratin, type i cytoskeletal 14 Antibody, NFJ Antibody
Host:	KRT14 antibody was produced in Mouse
Antigen Species:	KRT14 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG3
Antigen Type:	Synthetic peptide
Immunogen:	KRT14 antibody was raised against a 15 amino acid synthetic peptide corresponding to the C-terminus of human keratin 14. Cellular Localization: Cytoplasmic.
Antibody Specificity:	Recognizes human keratin 14 at 50kD. Species cross-reactivity: Rat.
Antigen Modification:	C-Terminus
Predicted Species Reactivity:	Rat
Purification:	Protein G Column
Presentation:	PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA, before the addition of glycerol to 40%
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2443 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2443 was determined to be 1:50. Positive control: A431 cells, skin or squamous cell carcinoma.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (1:50), Western blot (WB) (1 - 2 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	125 µl
Concentration:	0.1 mg/ml