Proteins and Peptides
Keratin Antibody (LS-B2238)
- 貨號 Cat. No.
- LS-B2238
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2238 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C45746. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Keratin |
| Host: | Keratin antibody was produced in Mouse |
| Antigen Species: | Keratin antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antibody Clone Name: | C-11 |
| Antigen Type: | Cells |
| Immunogen: | Keratin antibody was raised against keratin-enriched preparation from human epidermoid carcinoma cell line A431 |
| Antibody Specificity: | Reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
| Predicted Species Reactivity: | Mammal |
| Purification: | Protein A Column |
| Presentation: | PBS, 15 mM sodium azide, approx., pH 7.4. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2238 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2238 was determined to be 10 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Immunocytochemistry (ICC), Western blot (WB), Immunoprecipitation (IP), Flow Cytometry (Flo) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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