Isopeptidase T (USP5)
- 貨號 Cat. No.
- E-320
- 規格 Size
- 10 μg 25 μg
- 品牌 Brand
- Boston Biochem
Description
Isopeptidase T (USP5)
rabbit
Product Information
10 μg 25 μg
X mg/ml (X μM) in 50 mM HEPES, 150 mM NaCl, 1mM DTT, pH 8.0 Actual concentration will vary with specific Lot #.
97 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support hydrolysis of substrates in vitro ranges from 10-100 nM depending on conditions and substrate. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-320
Isopeptidase T (USP5)
Product Source:
rabbit
Also known as: IsoT
Isopeptidase T is a member of the high molecular weight ubiquitin C-terminal hydrolases (UCHs) that are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters from the C-terminus of ubiquitin. This enzyme primarily disassembles free polyubiquitin chains linked through isopeptide bonds. This activity has a critical role in the recycling of Ub and in the regulation of the activity of the 26S proteasome.
Product Information
Quantity:
10 μg 25 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES, 150 mM NaCl, 1mM DTT, pH 8.0 Actual concentration will vary with specific Lot #.
MW:
97 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support hydrolysis of substrates in vitro ranges from 10-100 nM depending on conditions and substrate. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.