ISG15 AMC
- 貨號 Cat. No.
- UL-553
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
ISG15 AMC
human recombinant
Product Information
50 μg
X mg/ml (XμM) in 50 mM Hepes pH 6.5, 200 mM NaCl, 10 % glycerol. Actual concentration will vary with specific Lot #.
17 kDa
> 95% by HPLC
Use & Storage
Substrate concentrations for assay range from 0.1-1 μM. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: UL-553
ISG15 AMC
Product Source:
human recombinant
Fluorogenic substrate for ISG15 hydrolases based on the carboxy-terminus derivatization of ISG15 with 7-amido-4-methylcoumarin (AMC). ISG15 AMC is useful for studying enzymes (such as UBP43) when detection sensitivity or continuous monitoring of activity is essential.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (XμM) in 50 mM Hepes pH 6.5, 200 mM NaCl, 10 % glycerol. Actual concentration will vary with specific Lot #.
MW:
17 kDa
Purity:
> 95% by HPLC
Use & Storage
Use:
Substrate concentrations for assay range from 0.1-1 μM. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.