ISG15-agarose
- 貨號 Cat. No.
- UL-630
- 規格 Size
- 0.5 ml
- 品牌 Brand
- Boston Biochem
Description
ISG15-agarose
human recombinant
Product Information
0.5 ml
ISG15 coupled to agarose at 2.5 mg/ml resin (0.15 mM). Resin supplied in 50 mM Hepes pH 7.5, 250 mM NaCl.
Use & Storage
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5 ml 50 mM Tris pH 9.0, 1 M KCl. Resin should be stored at 4°C, and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE
Catalog #: UL-630
ISG15-agarose
Product Source:
human recombinant
ISG15 covalently coupled to agarose beads via primary amines allowing for a fully functional Cterminus. Useful for isolation and capture of ISG15 interacting proteins such as the ISG15 activating E1 enzyme, the ISG15 carrier enzyme Ubch8, ISG15 E3 ligases, UBP43 (USP18) and other proteins/enzymes that have an affinity for ISG15 protein.
Product Information
Quantity:
0.5 ml
Stock:
ISG15 coupled to agarose at 2.5 mg/ml resin (0.15 mM). Resin supplied in 50 mM Hepes pH 7.5, 250 mM NaCl.
Use & Storage
Use:
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.
Storage:
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5 ml 50 mM Tris pH 9.0, 1 M KCl. Resin should be stored at 4°C, and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE