ISG15 Activating Enzyme (E1)
- 貨號 Cat. No.
- E-309
- 規格 Size
- 25 μg
- 品牌 Brand
- Boston Biochem
Description
ISG15 Activating Enzyme (E1)
human reconbinant
Product Information
25 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific Lot #.
112 kDa
> 90% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Store at –80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-309
ISG15 Activating Enzyme (E1)
Product Source:
human reconbinant
Also known as: UBA7
This E1 is responsible for the first step in ISG15-protein isopeptide bond formation. This enzyme catalyzes the activation of the C-terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner. The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade. The E1 enzyme is a critical component for the initiation of any in vitro conjugation reactions.
Product Information
Quantity:
25 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific Lot #.
MW:
112 kDa
Purity:
> 90% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Storage:
Store at –80°C. Avoid multiple freeze/thaw cycles.