IP-10/CXCL10 (Mouse) ELISA Kit

Alternate Name : C7, IFI10, INP10, SCYB10, crg-2, gIP-10, mob-1

Tag Line :

A Sandwich ELISA kit for the quantitative measurement of mouse IP-10/CXCL10 in plasma, tissue homogenates and other biological fluids.

Storage Conditions: 4ºC

Shelf life: 6 months

Summary :

Detection Method: Sandwich ELISA, Absorbance (450 nm)

Features & Benefits:

Precision: Coefficient of variation is < 10%,Detection Range: 31.25-2000 pg/mL,Sensitivity: 18.75 pg/mL,Specificity: This kit recognizes Mouse IP-10/CXCL10 in samples. No Significant cross-reactivity or interference between Mouse IP-10/CXCL10 and analogues was observed.

Description:

Chemokine (C-X-C motif) ligand 10 (CXCL10), also known as Interferon gamma-inducible protein-10 (IP-10), is a non-ELR chemokine secreted by various cell types including monocytes, endothelial cells, and fibroblasts in response to IFN-γ. CXCL10 functions via chemokine receptor CXCR3. CXCL10 has been attributed to several roles, such as chemo-attraction for activated T-lymphocytes, inhibition of angiogenesis, and antitumor activity. BioVision’s IP-10/CXCL10 (Mouse) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IP-10/CXCL10. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IP-10/CXCL10 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse IP-10/CXCL10. The concentration of Mouse IP-10/CXCL10 in the samples can be calculated by comparing the OD of the samples to the standard curve.