Intra Acrosomal Protein antibody [ACR-4]

Product Name 

Intra Acrosomal Protein antibody [ACR-4]

Product type 

Primary antibodies

Description 

Mouse monoclonal [ACR-4] to Intra Acrosomal Protein

Immunogen 

Tissue / cell preparation (Pig). Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for imunization.

Cow, Pig

ICC, WB

Recommended dilutions

ICC: Use at an assay dependent dilution.

WB: Use at an assay dependent dilution. Predicted molecular weight: 32 kDa.



Not tested in other applications.

Optimal dilutions/concentrations should be determined by the end user.

Relevance 

This protein is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The gene encoding this protein consists of 4 exons and its alternative splicing generates 11 distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining 7 transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

ACR-4

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 15mM Sodium Azide

Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:

Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.