Interleukin 31 (IL-31), ELISA Kit

貨號 Cat. No.: MBS740462-96Wells
規格 Size: 96-Strip-Wells
品牌 Brand: MyBioSource

Description

Product Name	Interleukin 31 (IL-31), ELISA Kit â˜…Popular Itemâ˜…
Also Known As	Canine Interleukin 31 ELISA Kit
Product Gene Name	IL-31 elisa kit	[Similar Products]
Research Use Only	For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
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MBS740462 MSDS

OMIM	609509

Species Reactivity	Canine

Specificity	This assay has high sensitivity and excellent specificity for detection of IL-31. No significant cross-reactivity or interference between IL-31 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-31 and all the analogues, therefore, cross reaction may still exist in some cases.

Samples	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type	Quantitative Competitive
Sensitivity	1.0 pg/mL.

Preparation and Storage	Store all reagents at 2-8 degree C.
Sample Preparation	We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note	Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes	Small volumes of IL-31 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IL-31 purchase	MBS740462 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 31 (IL-31) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-31, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS740462 kit is based on IL-31 antibody-IL-31 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-31 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-31. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-31 elisa kit	Intended Uses: This IL-31 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IL-31. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: IL-31 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-31 antibody and an IL-31-HRP conjugate. The assay sample and buffer are incubated together with IL-31-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-31 concentration since IL-31 from samples and IL-31-HRP conjugate compete for the anti-IL-31 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-31 from the sample, fewer sites are left to bind IL-31-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-31 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IL-31 elisa kit	Immunology