Proteins and Peptides
Insulin Antibody (LS-B1445)
- 貨號 Cat. No.
- LS-B1445
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B1445 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C18823. It has been validated for use in the following assays: IHC. |
| Antibody Target: | Insulin (INS) |
| Synonyms: | INS, insulin |
| Family: | Hormone |
| Subfamily: | not assigned-Hormone |
| Host: | INS antibody was produced in Mouse |
| Antigen Species: | INS antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1,k |
| Antibody Clone Name: | 2D11.H5 |
| Antigen Type: | Fusion protein |
| Immunogen: | INS antibody was raised against fusion protein |
| Antibody Specificity: | Purified human insulin coupled to bovine serum albumin (BSA). Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with insulin from human pancreas using conventional hybridoma technology. |
| Purification: | Protein A Column |
| Presentation: | 0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B1445 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1445 was determined to be 5 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB), Immunoprecipitation (IP), ELISA (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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