Insig1 Antibody
- 貨號 Cat. No.
- 3854-100
- 規格 Size
- 100 µg
Description
pstrongAntibody Target :/strongInsig1/p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic peptide surrounding amino acid 248 of rat Insig1/p
pbAccession#: /bNP_071787/p
pbGene ID: /b64194/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/rt/bv/hst/p
pbApplication: /bWB/p
pbApplication amp; Usage: /bWestern blotting (0.5-4 micro;g/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~30 kDa InSig-1 and its precursor (~50 kDa) in samples from human, mouse and rat origins. Reactivity to other species has not been tested./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bInsulin-induced gene (InSig) localizes in the endoplasmic recticulum(ER) and is highly expressed in the liver and fibroblast cell lines. InSig1 and InSig2 play important roles in the regulation of cholesterol biosynthesis. Sterol induces InSig1 binding to the sterol-sensing domain of SREBP cleavage-activating protein (SCAP). Both InSig1 and Insig2 prevent the export of SCAP from the ER and thus, inhibit cholesterol synthesis by preventing the proteolytic cleavage of SREBPs by the Golgi enzymes./p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic peptide surrounding amino acid 248 of rat Insig1/p
pbAccession#: /bNP_071787/p
pbGene ID: /b64194/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/rt/bv/hst/p
pbApplication: /bWB/p
pbApplication amp; Usage: /bWestern blotting (0.5-4 micro;g/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~30 kDa InSig-1 and its precursor (~50 kDa) in samples from human, mouse and rat origins. Reactivity to other species has not been tested./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bInsulin-induced gene (InSig) localizes in the endoplasmic recticulum(ER) and is highly expressed in the liver and fibroblast cell lines. InSig1 and InSig2 play important roles in the regulation of cholesterol biosynthesis. Sterol induces InSig1 binding to the sterol-sensing domain of SREBP cleavage-activating protein (SCAP). Both InSig1 and Insig2 prevent the export of SCAP from the ER and thus, inhibit cholesterol synthesis by preventing the proteolytic cleavage of SREBPs by the Golgi enzymes./p