iNOS (D6B6S) Rabbit mAb
Antibodies
- 貨號 Cat. No.
- 13120S
- 規格 Size
- 100 µl
- 品牌 Brand
- Cell Signaling Technology
- Reactivity
- Mouse
Description
Product Usage Information
Application | Dilution |
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Flow Cytometry | 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
iNOS (D6B6S) Rabbit mAb recognizes endogenous levels of total iNOS protein. This antibody does not cross-react with other NOS proteins.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1133 of mouse iNOS protein.
Background
Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).
Nitric oxide catalyzed by iNOS is involved in host defense against protozoa, bacteria, fungi and viruses. Unlike constitutively expressed eNOS and nNos, iNOS is not usually expressed in quiescent cells. iNOS is transcriptionally induced in response to bacterial endotoxins, such as LPS and proinflammatory cytokines, in macrophages and various other cell types. Transcription factors involved in iNOS transcription include NF-κB, AP-1 and STAT. Different signaling pathways either promote (Jak1/2, PKC, c-Raf, p38 MAP kinase and p44/42 MAP kinase) or inhibit (PI3 kinase) iNOS expression depending on stimulus and cell type (4).
- Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.
- Son, H. et al. (1996) Cell 87, 1015-23.
- Hawkins, R.D. (1996) Neuron 16, 465-467.
- Bogdan, C. (2001) Nat Immunol 2, 907-16.
