Influenza A H1N1 Hemagglutinin / HA Antibody, Mouse MAb_11684-MM03

分子 : HA

 

保存條件 :

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant H1N1 (A/Puerto Rico/8/1934) HA (Catalog#11684-V08H; ABD77675.1; Met 1-Gln 528). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

 

抗原貨號 : 11684-V08H

 

抗原描述 : Recombinant H1N1 (A/Puerto Rico/8/1934) HA protein (Catalog#11684-V08H)

 

CloneID : 2F1A7

 

Ig類型Cross Reactivity : Mouse IgG1

 

纯化方法 : Protein A

 

製備方法 :

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant H1N1 (A/Puerto Rico/8/1934) HA (Catalog#11684-V08H; ABD77675.1; Met 1-Gln 528). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

 

特異性 : H1N1 (A/Puerto Rico/8/1934) HA

 

有交叉ELISA :

 

無交叉ELISA : No cross-reactivity in ELISA with
H1N1 (A/California/07/2009) HA
H1N1 (A/California/04/2009) HA
H1N1 (A/California/04/2009) HA1
H1N1 (A/Brisbane/59/2007) HA
H1N1 (A/Brisbane/59/2007) HA1
H1N1 (A/BrevigMission/1/1918) HA
H1N1 (A/Solomon Islands/3/2006)
H1N1 (A/Ohio/UR06-0091/2007) HA
H1N1 (A/New Caledonia/20/1999) HA
H1N1 (A/WSN/1933) HA
H1N2 (A/swine/Guangxi/13/2006) HA
H1N3 (A/duck/NZL/160/1976)
H5N1 (A/Anhui/1/2005) HA
H5N1 (A/Anhui/1/2005) HA2

 

有交叉IHC :

 

無交叉IHC :

 

内毒素 :

 

緩冲液 : 0.2 μm filtered solution in PBS

 

濃度 :

 

血凝抑制 :

 

WB推薦濃度 : This antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect H1N1 (A/Puerto Rico/8/1934) HA in WB. Using a DAB detection system, the detection limit for H1N1 (A/Puerto Rico/8/1934) HA is approximately 8 ng/lane under non-reducing conditions.Use of this antibody under reducing conditions is not recommended.

 

ELISA推薦濃度 : ELISA: 1:1000-1:2000

 

IHC_P推薦濃度 :

 

IHC_Fr推薦濃度 :

 

ICC_IF推薦濃度 :

 

FCM推薦濃度 :

 

IP推薦濃度 :

 

Sandwich_ELISA推薦濃度 : ELISA(Det): 1:1000-1:10000