Proteins and Peptides
Influenza A H1N1 (A/California/07/2009) Hemagglutinin
- 貨號 Cat. No.
- 11085-V08H
- 規格 Size
- 100µg
- 品牌 Brand
- Sino Biological
Description
Influenza A H1N1 ( A/California/7/2009 ) Hemagglutinin / HA Protein
H1N1 ( A/California/7/2009 ) HA Protein Product Information
| Synonym: | HA |
| Protein Construction: | A DNA sequence encoding the extracellular domain of Influenza A virus H1N1 (A/California/07/2009) (ACP44189.1) hemagglutinin (Met 1-Gln 529) (HA1+HA2, two amino acids deletion, uncleaved) was fused with a polyhistidine tag at the C-terminus. |
| Source: | Influenza A Virus H1N1 |
| Expression Host: | Human Cells |
H1N1 ( A/California/7/2009 ) HA Protein QC Testing
| Purity: | > 95 % as determined by SDS-PAGE | SDS-PAGE: H1N1 HA protein |
| Endotoxin: | < 1.0 EU per μg of the protein as determined by the LAL method. | |
| Stability: | Samples are stable for up to twelve months from date of receipt at -70℃ | |
| Predicted N terminal: | Asp 18 | |
| Molecular Mass: | The secreted recombinant hemagglutinin of Influenza A virus (A/California/07/2009(H1N1)) comprises 521 amino acids with the predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 75-85 kDa band in SDS-PAGE under reducing conditions. | |
| Formulation: | Lyophilized from sterile PBS, pH 7.4
|
H1N1 ( A/California/7/2009 ) HA Protein Usage Guide
| Storage: | Store it under sterile conditions at -70℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution: | A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. |
H1N1 ( A/California/7/2009 ) HA Related Influenza Virus Research Tools
- Influenza Virus Research Tools
- Influenza Hemagglutinin & Antibody
- Influenza Hemagglutinin Proteins
- H1N1 Hemagglutinin & Antibody
- H1N1 Hemagglutinin Proteins
- A/California/7/2009 (A/California/7/09)
H1N1 HA Protein Description
Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.
References
- Günther-Ausborn, S. et al., 2000, J. Virol. 74: 2714-2720.
- Hoffman, LR. et al., 1997, J. Virol. 71: 8808-8820.
- Marjuki, H. et al., 2006, J. Biol. Chem. 281: 16707-16715.
- Gething, M-J. et al., 1980, Nature. 287: 301-306.
- Naeve, C.W. and Williams, D. 1990, EMBO. J. 9: 3857-3866.
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