IL-6 Protein, Human, Recombinant

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Activity

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 μg/ml (100 μl/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/mL.

2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.75-3 ng/mL. The specific activity of Recombinant Human IL-6 is approximately 0.35 × 105 IU/μg.

Protein Construction

A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val 30-Met 212) was expressed, with an initial Met at the N-terminus.

Accession#

NP_000591.1

Expressed Host

E. coli

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human IL6 consists of 184 amino acids and migrates with an apparent molecular mass of 20.3 kDa as estimated in SDS-PAGE under reducing conditions.