IL-6 (human) ELISA Kit

Alternate Name :

Tag Line :

Sensitive, Colorimetric Assay

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- human
• Application- Quantitative protein detection, establishing normal range etc.

Detection Method: Absorbance (450 nm)

Features & Benefits:

• Easy, convenient and time-saving method to assay for human IL-6.
• Detection Range: 4.69 pg/ml – 300 pg/ml. Sensitivity: < 0.3 pg/ml.
• This ELISA kit shows no cross-reactivity with other relevant proteins.

Description:

BioVision’s human IL-6 ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for human IL-6 coated on a 96-well plate. Standards (E. coli P29-M212) and test samples are added to the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human IL-6 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 4.69 pg/ml – 300 pg/ml. Sensitivity: < 0.3 pg/ml.