IL-4 Protein, Mouse, Recombinant

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Activity

Measured in a cell proliferation assay using HT-2 mouse T cells.

The ED50 for this effect is typically 0.4-2 ng/mL.

Protein Construction

A DNA sequence encoding the mouse IL4 (NP_067258.1) (His23-Ser140) was expressed with a N-terminal Met.

NP#

Expressed Host

E. coli

Species

Mouse

Predicted N Terminal

Met

Molecule Mass

he recombinant mouse IL4 consists of 119 amino acids and predicts a molecular mass of 13.4 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4.

1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

2. Please contact us for any concerns or special requirements.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -70℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.