IL-3R (F587) Antibody

貨號 Cat. No.

BS1185

規格 Size

100μg

品牌 Brand

Bioworld

Description

stock

Host: Rabbit

Reactivity: Human,Mouse,Rat

Product

1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Purification & Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity

IL-3R (F587) antibody detects endogenous levels of IL-3R protein.

Applications (Recommended Dilutions)

WB: 1:500~1:1000 IHC: 1:50~1:200

Western Blot(WB)

Western blot (WB) analysis of IL-3R (F587) antibody in extracts from RAW264.7 cells treated with G-CSF 25ng/ml 15'.

Immunohistochemistry (IHC)

Immunohistochemistry (IHC) analyzes of IL-3R (F587) antibody in paraffin-embedded human brain tissue.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background

The human IL-3, IL-5 and GM-CSF receptors are each composed of both unique ??subunits and a common 130 kDa ?subunit. The ??subunits are low affinity ligand binding proteins while the ??subunits do not themselves bind ligand, but are required for high affinity binding by the ??subunits. In contrast, the mouse IL-3 receptor has two distinct ??subunits, one that functions only in IL-3 mediated cell signaling and a second that is shared with IL-5 and GM-CSF. The murine -subunits are 91% homologous at the amino acid level but only 56% homologous to the human ??subunit. Although neither the murine nor the human ??subunit contains tyrosine kinase domains, both activate tyrosine phosphorylation mediated signaling pathways

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