IL-24 (Mouse) ELISA Kit

Alternate Name :

Tag Line :

A Sandwich ELISA kit for the quantitative measurement of mouse IL-24 in human serum, plasma and other biological fluids.

Storage Conditions: 4ºC

Shelf life: 6 months

Summary :

Detection Method: Sandwich ELISA, Absorbance (450 nm)

Features & Benefits:

Specificity: No Significant cross-reactivity or interference between Mouse IL-24 and analogues was observed.,Detection Range: 31.25-2000 pg/mL,Sensitivity: 18.75 pg/mL,Precision: Coefficient of variation is < 10%

Description:

IL-24 is induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by IL-24 in melanoma cells, but not in normal immortal melanocytes. BioVision’s IL-24 (Mouse) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-24. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-24 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-24 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse IL-24. The concentration of Mouse IL-24 in the samples can be calculated by comparing the OD of the samples to the standard curve.