IL-10, human recombinant

Alternate Names / Synonyms: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

Gene Symbol: IL10

Accession #: P22301

Gene ID: 3586

Gene Source: Human

Recombinant Host: E. coli

Appearance: Lyophilized protein

Physical Form: Lyophilized from PBS

Molecular Weight: 18.6 kDa

Purity by SDS-PAGE: ≥98%

Purity by HPLC: ≥98%

Endotoxin Level: <0.1 ng/μg

Biological Activity: The EDâ‚…â‚€ determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells is < 2 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.

Reconstitution: Reconstitute in water a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Storage Temp.: -20°C

Shipping: Gel pack

Background Information: IL-10 (Interleukin-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.6 kDa protein containing 161 amino acid residues. It shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production.

Handling: Centrifuge the vial prior to opening.