Proteins and Peptides
IGF1R Antibody (LS-B2905)
- 貨號 Cat. No.
- LS-B2905
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2905 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C7533. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | IGF1R |
| Synonyms: | IGF1R Antibody, CD221 Antibody, IGF1 Receptor Antibody, igfIr Antibody, IGFR Antibody, insulin-like growth factor 1 receptor Antibody, insulin-like growth factor i receptor Antibody, insulin-like growth factor-1 receptor Antibody, JTK13 Antibody, MGC142170 Antibody, MGC142172 Antibody, MGC18216 Antibody, type 1 IGF receptor Antibody, type 1 insulin-like growth factor receptor Antibody |
| Family: | Protein Kinase |
| Subfamily: | Insulin receptor |
| Host: | IGF1R antibody was produced in Mouse |
| Antigen Species: | IGF1R antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG2a |
| Antigen Type: | Fusion protein |
| Immunogen: | IGF1R antibody was raised against gST fusion protein corresponding to residues 1264-1367 of human IGF-1 Receptor. |
| Antibody Specificity: | Recognizes human IGF-1 receptor beta chain, MW 95kD. Recognizes transmembrane domain. Does not cross-react with insulin receptor. Species cross-reactivity: Mouse. |
| Antigen Modification: | aa1264-1367 |
| Predicted Species Reactivity: | Mouse |
| Purification: | Protein A Column |
| Presentation: | Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 40% |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2905 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2905 was determined to be 5 ug/ml. Western Blot: 0.1-2 ug/ml. Detects the beta chain of IGF1R in RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells. C4 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with i7661-18. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug/ml immunoprecipitates IGF1R from 1 mg of RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF1R) and in RIPA lysates from mouse 3T3/A31 cells. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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