IEGF1 protein
- 貨號 Cat. No.
- IEGF1 protein
- 規格 Size
- 100ug
- 品牌 Brand
- gene-rl
Description
| Product Name | IEGF1 protein |
| Product type | Proteins and Peptides |
| Size | 100ug |
| Peptide | Recombinant full length protein, with a N-terminal hexahistidine tag. |
| Tested applications | SDS-PAGE, Western Blotting, Cell Test |
| Application notes | Recommended dilutions SDS-PAGE: Use at an assay dependent dilution Western Blotting: Use at an assay dependent dilution Optimal dilutions/concentrations should be determined by the end user. |
| Relevance | Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth. |
| Storage buffer | n/a |
| Purity | >95% by SDS-PAGE and PLC analysis. |
| Form | Lyophilised |
| Storage instructions | Aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Expression system | Yeast (Eukaryotic cell) |
| Sequence | Human IGF1 is a 7.6 kDa protein containing 70 amino acid residues: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
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0 ng/ml | 200 ng/ml | 2 ug/ml | 200 ug/ml | ||||||
| Fig 1: IGF Treatment of 3T3 Cell Line 　　Different concentration of purified IGF was added into 3T3 cell culture medium. 　　Pictures were taken after 24 or 48 hours. 　　The purified growth factor increased the proliferation of 3T3 cell line. ã€€ã€€ç”Ÿé•·å› å在3T3的細胞ä¸ç”¨ä¸åŒæ¿ƒåº¦åŸ¹é¤Š,æ–¼24或48å°æ™‚æ‹ç…§è§€å¯Ÿ ã€€ã€€ç”Ÿé•·å› åå¯å¢žåŠ 3T3分化生長的能力 | |||||||||
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