ICAM-1 Antibody (LS-B2647)

貨號 Cat. No.: LS-B2647
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2647
Antibody Validation:	This antibody replaces catalog number LS-C13762. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Intercellular Adhesion Molecule 1 (ICAM1)
Synonyms:	ICAM1, BB2, cd54, icam-1, intercellular adhesion molecule-1, major group rhinovirus receptor, major group rhinovirus receptors, P3.58
Family:	ICAM
Subfamily:	not assigned-ICAM
Host:	ICAM1 antibody was produced in Mouse
Antigen Species:	ICAM1 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1
Antigen Type:	Cells
Immunogen:	ICAM1 antibody was raised against human monocytes.
Antibody Specificity:	Recognizes the human CD54 cell surface antigen, a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators. Clone 3H1547 blocks CD54 function.
Purification:	Protein G Column
Presentation:	PBS, pH 7.4, 0.09% sodium azide
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2647 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2647 was determined to be 10 ug/ml. Flow Cytometry: 1:50-1:100. Use 10 ul to label 10^6 cells in 100ul. Positive control: Human Tonsil.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Immunohistochemistry - Frozen (IHC-Fr) (1:50 - 1:100), Immunoprecipitation (IP), Flow Cytometry (Flo) (1:50 - 1:100) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml