δ-Dendrotoxin
- 貨號 Cat. No.
- D-380
- 規格 Size
- 70 μg 5x70 μg
Description
d-Dendrotoxin
A Potent and Specific Blocker of Kv1.1 Channels
| Product#: | D-380 |
| Sizes: |
| 70 mg |
| 5 x 70 mg |
| M.W.: | 6573 |
| Sequence: | AAKYC KLPVR YGPCK KKIPS FYYKW KAKGC LPFDY SGCGG NANRF KTIEE CRRTC VG |
| Purity: | >98% |
| Bioassay: |
d-Dendrotoxin was potent in blocking Kv1.1 channels (92 3%, n=4), but blocked only a relatively small fraction (20%, n=2) of cloned Kv1.2 channels, both expressed in Xenopus oocytes. |
 |
| Kv1.1 (left, in 2mM K+) and Kv1.2 (right, in 5mM K+) channel currents, elicited by 200 ms depolarization from holding potential of ?00 mV to +20 mV, before and during application of 100 nM d-Dendrotoxin. |
| References: |
1. | Benishin, C.G. et al. (1988) Mol. Pharmacol. 34, 152. |
2. | Harvey, A.L. and Anderson A.J. 1985) Pharmacol. Ther. 31, 33. |
3. | Imredy, J.P. et al. (1998) Biochemistry 37, 14876. |
4. | Harvey, A.L. (2001) Toxicon 39, 15. |
d-Dendrotoxin is isolated from Dendroaspis angusticeps1 snake venom, by modification of the procedures of Harvey2 and Benishin1 and purified to homogeneity.
d-Dendrotoxin blocks Kv1.1 channels (IC50= 0.1 nM,3 for review see4). The toxin was shown to block also Kv1.6 (IC50= 23 nM).4 In addition d-Dendrotoxin blocks ROMK1 inward rectifier K+ channels with Kd of 150 nM.3