Human Whole Blood Monocyte Isolation Kit

Alternate Name :

Tag Line :

Simple, one step assay to isolate monocytes

Storage Conditions: 4 °C

Shelf life: 12 months

Summary :

• Isolation of Monocytes from human whole blood.
• Determination of cell viability and also contamination from RBCs in isolated cell fraction.

Detection Method:

Features & Benefits:

• PBMC high yields (≥5 x106 cells/ml)
• By using the Viability Stain, it was proven that 99% of the cells are viable and the isolated fraction contains low red blood cell counts (≤ 3%)

Description:

Monocytes are a type of white blood cell (leukocyte) and the largest of the immune cells. They are produced in the bone marrow and then migrate to the blood stream where they comprise 3-8% of all leukocytes. Depending on the clincial presentation of disease, monocytes will migrate to a specific tissue and then differentiate into a tissue-specific dendritic cell or a macrophage. Monocytes are easily identified by the large amoeboid shape, unilobar nucleus and vacuolated cytoplasm. They serve three important roles: phagocytosis, presentation of antigen to T cells which subsequently results in T cell clonal expansion, and the production of cytokines. BioVision’s human Whole Blood Monocyte Isolation Kit allows for in vitro isolation of intact, viable monocytes. The kit enables the isolation of ≥ 5 x106 monocytes/ml yielding approximately 80% of the total monocytes present in 1 ml of whole blood. The viability stain (included) used to identify living monocytes indicates that more than 99% of the cells in the isolated fraction are viable. The isolated fraction is pure - it contains more than 95% monocytes with less than 3% red blood cell counts.