Human VCAM1 ELISA Kit

分子 : VCAM1

 

抗原貨號 : 10113-H08H

 

產品介绍 : For the quantitative determination of Human VCAM1 concentration in serum, cell culture supernates and plasma.

 

線性範圍: 109.38-7000

 

包被抗體類型 : mouse mAb

 

檢測抗體類型 : mouse mAb

 

靈敏度: 29.51

 

天然樣本測定含量: The average concentration of Human VCAM1 in 10 normal human serum is 451.25 +/- 78.98 ng/mL ranging from 298.56 to 546.33 ng/mL.The average concentration of Human VCAM1 in 10 normal human plasma is 422.28 +/- 90.08 ng/mL ranging from 316.14 to 600.27 ng/mL.Human peripheral blood mononuclear cells （1 x 10E6 cells/mL） were cultured in RPMI supplemented with 10% fetal calf serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate, stimulated with 10 μ

 

特異性 : This assay recognizes both recombinant and natural Human VCAM1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

 

干擾 : Preparations of the factors listed below at 50 ng/m in a mid-range recombinant human VCAM1 control were assayed for interference. No significant interference was observed.

 

檢測原理: Solid Phase Sandwich ELISA

 

試劑盒组分 :

1. 96 well microplate coated with Capture Antibody

2. Detection Antibody conjugated to HRP

3. Standards

4. Wash Buffer Concentrate

5. Dilution Buffer Concentrate

6. Color Reagent A

7. Color Reagent B

8. Stop Solution