Human UCH-L3 / UCHL3 Protein, His Tag

Source : 

Human UCH-L3, His Tag (UC3-H5141) is expressed from E.coli cells. It contains AA Glu 2 - Ala 230 (Accession # P15374-1).

 

Molecule : UCH-L3

 

Synonyms : UCHL3,Ubiquitin thioesterase L3

 

Format : Powder

 

Category : Enzymes

 

Accession : AAH18125.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 27.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 27.0 kDa. The protein migrates as 27 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM NaCl, pH7.5

 

Description :

Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCH-L3), a member of peptidase C12 family, is also known as ubiquitin thioesterase L3. Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. UCH-L3 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is also required for stress-response retinal, skeletal muscle and germ cell maintenance. Furthermore, UCH-L3 may be involved in working memory and can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.

 

References :

(1) Nam M.J., et al., 2003, Proteomics 3:2108-2115.
(2) Setsuie R., et al., 2009, Neurochem. Int. 54:314-321.
(3) Iphofer A., et al., 2012, ChemBioChem 13:1416-1420.