Human UCH-L1 Protein, His Tag

Source : 

Human UCH-L1, His Tag (UC1-H5140) is expressed from E.coli cells. It contains AA Gln 2 - Ala 223 (Accession # NP_004172).

 

Molecule : UCH-L1

 

Synonyms : UCHL1,PGP9.5

 

Format : Powder

 

Category : Other Recombinant Proteins

 

Accession : NP_004172

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 25.7 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 25.7 kDa. The protein migrates as 27-28 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM Tris, 150 mM Nacl, pH8.0

 

Description :

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ,a member of the peptidase C12 family, is also known as neuron cytoplasmic protein 9.5 (PGP 9.5) and ubiquitin thioesterase L1.About 30% of total UCHL1 is associated with membranes in brain. UCHL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also,UCHL1 can bind to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. Furthermore, the mutations of UCHL1 gene can result in parkinson disease 5.

 

References :

(1) Choi J., et al., 2004, J. Biol. Chem. 279:13256-13264.
(2) Liu Y., et al., 2002, Cell 111:209-218.
(3) Choudhary C., et al., 2009, Science 325:834-840.