Human TrkB / NTRK2 Protein, His Tag

Source : 

Human TrkB, His Tag (NT2-H5228) is expressed from human 293 cells (HEK293). It contains AA Cys 32 - His 430 (Accession # AAH31835).

 

Molecule : TrkB

 

Synonyms : NTRK2,TRKB,GP145-TrkB

 

Format : Powder

 

Category : Bio-Markers & CD Antigens

 

Accession : AAH31835

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 45.2 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 45.2 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Neurotrophic tyrosine kinase receptor type 2 (NTRK2) is also known as BDNF/NT-3 growth factors receptor, Tropomyosin-related kinase B (TRKB) and TrkB tyrosine kinase, which belongs to the protein kinase superfamily or Tyr protein kinase family. Insulin receptor subfamily. NTRK2 / TrkB contains two Ig-like C2-type (immunoglobulin-like) domains, two LRR (leucine-rich) repeats, one LRRCT domain, one LRRNT domain, one protein kinase domain. NTRK2 / Trk-B is expressed in the central and peripheral nervous system. The catalytic activity of NTRK2 is “ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate”. NTRK2 / TrkB involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity.

 

References :

(1) Allen S.J., et al., 1994, Neuroscience 60:825-834.
(2) Stoilov P., et al., 2002, Biochem. Biophys. Res. Commun. 290:1054-1065.
(3) Meakin S.O., et al., 1999, J. Biol. Chem. 274:9861-9870.
(4) Yeo G.S., et al., 2004, Nat. Neurosci. 7:1187-1189.