Human TREM2 ELISA Kit (1 Plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Triggering receptor expressed on myeloid cells 2; TREM-2; Triggering receptor expressed on monocytes 2; TREM2

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

ELISA. The purpose of this kit is the in-vitro quantitative determination of human TREM2 in samples such as sera, plasma (heparin, EDTA), and cell culture supernates. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : The protein Triggering receptor expressed on myeloid cells 2 (TREM2) forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein (TYROBP). TREM2 has roles in the immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding isoforms with differing subcellular locations.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1674

 

Description (Long Description) : 

The human TREM2 kit is a sandwich ELISA. The capture antibody is a mouse monoclonal TREM2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TREM2 concentration are added to these wells and allowed to complex with the bound TREM2 antibody. A biotinylated goat polyclonal TREM2 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TREM2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TREM2 in samples such as sera, plasma (heparin, EDTA), and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

6 months after purchase if unopened

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen for this kit is recombinant TREM2 protein produced in and purified from an E.coli expression system using immunogen sequence H19-E167.

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :http://www.biosensis.com/pdf_MSDS.php?products_id=1674