Human TRAIL / TNFRSF10 Protein, Mouse IgG2a Fc Tag

Source : 

Human TRAIL, Mouse IgG2a Fc Tag (TRL-H5259) is expressed from human 293 cells (HEK293). It contains AA Val 114 - Gly 281 (Accession # P50591-1).

 

Molecule : TRAIL

 

Synonyms : Apo-2L,TRAIL,CD253,TNFSF10,Apo-2 ligand

 

Format : Powder

 

Category : Cytokines&Growth Factors

 

Accession : NP_003801.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 46.4 kDa

 

Characteristics :

This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 46.4 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 0.1 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

TRAIL, also known as TNFSF10, APO2L, Apo-2L, CD253, TL2, TRAIL, TNLG6A and TNF superfamily member 10. The TRAIL gene as a drug target, induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). DcR1 functions as a TRAIL-neutralizing decoy-receptor. The cytoplasmic domain of DcR2 is functional and activates NFkappaB. In cells expressing DcR2, TRAIL binding therefore activates NFkappaB, leading to transcription of genes known to antagonize the death signaling pathway and/or to promote inflammation.

 

References :

(1) Song JJ, et al. 2008. Cell Signal. 20(5):892-906.
(2) Ramamurthy V, et al. 2015. Acta Crystallogr F Struct Biol Commun. 71(Pt 10):1273-81.
(3) Hymowitz SG, et al. 1999. Mol Cell. 4(4):563-71.